(Source: Wikipedia, plus minor edits by Espionne)
Totally Spies! features the adventures and missions of three teenage spies (Sam, Alex and Clover) led by the founder and administrator of WOOHP, Jerry. The surnames of Clover and Alex are unknown.
- Samantha "Sam" – (Seasons 1–6, including the movie): Sam is the unofficial leader of the trio. She has long, wavy orange hair and green eyes, and wears a green catsuit. She is the most intelligent, responsible, and practical of the girls. She is serious about academics, enjoys reading, and excels in martial arts. Her favorite colors are green and purple according to the episode "The Granny". Sam can play the accordion. Her mother's name is Gabby, short for Gabrielle. Gabby is portrayed as being almost identical to Sam in seasons 3-5.
- Alexandra "Alex" – (Seasons 1–6, including the movie): The youngest of the three, Alex has short black hair styled in a bob. She is half Caucasian from her dad's side and half black from her mom's side, and wears a yellow catsuit. She has brown eyes. Despite her clumsiness, Alex excels in athletics — particularly in soccer. She is funny and is always there for her friends. Though she is somewhat of a tomboy compared to Clover and Sam, Alex is just as eager when it comes to fashion and boys. She has a toy turtle named Ollie, whom she admits is her closest friend in the episode "Truth or Scare". Her favorite color is yellow according to the two-part episode "So Totally Not Spies". Her mom, Carmen, is almost identical to her in seasons 3-5. Alex became jealous of a new spy, Britney, in Season 2's Episode 44 ("Alex Quits"). Although she continues to deny her jealousy, she still shows hints of it when Britney returns in later episodes.
- Clover – (Seasons 1–6, including the movie): Clover is the most fashionable of the spies. She has medium-short blonde hair with pretty blue eyes, and wears a red catsuit. Clover is a boy-crazy shopaholic and fashion fanatic. She keeps up to date with the latest trends and always makes sure she looks good. Mandy is her rival and they're always fighting about the simplest things. She goes through many boys in the show, and she cares deeply about fashion and designer paraphernalia. Her mom's name is Stella and they are almost identical in seasons 3-5. Clover sometimes comes across as rude, but is always there for her friends.
- Gerald "Jerry" Lewis – (Seasons 1–6, including the movie): He is the founder and administrator of W.O.O.H.P. (World Organization of Human Protection). He is a middle-aged British gentleman who takes his work seriously. He has many different ways of transporting the girls ("WOOHPing" them) to his headquarters, even if it upsets them. He also briefs them on their missions, distributes their gadgets, and provides mission support, either with information or direct involvement. He often annoys the girls with his attitude and constant missions, but when he is in danger, the girls are always there to help. It is also known that he has trouble keeping his spy identity a secret from his nosy mother, who has only been mentioned a few times during the show. Sam and Alex once noted in the episode "Evil Sorority" that they don't listen to anyone but Jerry, and usually they don't even listen to him.